Journal: Advanced Science

Article Title: Targeting ATAD3A Phosphorylation Mediated by TBK1 Ameliorates Senescence‐Associated Pathologies

doi: 10.1002/advs.202404109

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Article Snippet: Mouse Tumor Necrosis Factor α (TNFα) ELISA Kit , Elabscience , Cat# E‐EL‐M3063.

Techniques: Ubiquitin Proteomics, Recombinant, Protease Inhibitor, Mutagenesis, Isolation, Staining, Membrane, One Step RT-PCR, Enzyme-linked Immunosorbent Assay, Software, Microscopy, Transmission Assay, Electron Microscopy

Journal: Cell chemical biology

Article Title: Dual Modifications of an α-Galactosyl Ceramide Synergize to Promote Activation of Human Invariant Natural Killer T Cells and Stimulate Anti-Tumor Immunity

doi: 10.1016/j.chembiol.2018.02.009

Figure Lengend Snippet: A) Enhanced plasma membrane lipid raft association of CD1d proteins presenting 4′-deoxyphytosphingosine variant AH10-7. Mouse JAWS II dendritic cells were preincubated with glycolipids to allow loading of CD1d molecules and then labeled by cell surface binding of fluorochrome conjugated mCD1d/α-GalCer complex-specific mAb L363. Cells were then treated with 0.05% Triton X-100, and elution of cell surface CD1d/glycolipid complexes was monitored over time by flow cytometry. Starting values for MFI were normalized to 100%, and the change in MFI values over time are plotted for cells loaded with KRN7000 and the other indicated glycolipids. Values at 32.5 seconds post detergent addition (vertical dashed lines) for a minimum of four replicate samples were averaged, and the mean ± 1 SE for percent residual MFI is plotted in the bar graph on the right. Results shown are representative of three separate experiments.

Article Snippet: The JAWS II cell line (derived from C57BL/6.p53 −/− mice) was obtained from the American Type Culture Collection (ATCC) and was cultured at 37°C in a 5% CO 2 incubator in α-MEM medium supplemented with 20% fetal calf serum (Atlanta Biologicals), 10 mM HEPES, 50 μM β-mercaptoethanol, 50 μg/ml gentamicin, 100 ng/ml GM-CSF (Peprotech), 0.1 mM of nonessential amino acids (NEAA) and essential amino acids (EAA).

Techniques: Clinical Proteomics, Membrane, Variant Assay, Labeling, Binding Assay, Flow Cytometry

Journal: Cell chemical biology

Article Title: Dual Modifications of an α-Galactosyl Ceramide Synergize to Promote Activation of Human Invariant Natural Killer T Cells and Stimulate Anti-Tumor Immunity

doi: 10.1016/j.chembiol.2018.02.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The JAWS II cell line (derived from C57BL/6.p53 −/− mice) was obtained from the American Type Culture Collection (ATCC) and was cultured at 37°C in a 5% CO 2 incubator in α-MEM medium supplemented with 20% fetal calf serum (Atlanta Biologicals), 10 mM HEPES, 50 μM β-mercaptoethanol, 50 μg/ml gentamicin, 100 ng/ml GM-CSF (Peprotech), 0.1 mM of nonessential amino acids (NEAA) and essential amino acids (EAA).

Techniques: Enzyme-linked Immunosorbent Assay, Virus, Recombinant, Transfection, Plasmid Preparation, Software